![]() Luer 4-way stopcocks, at least 5 (Cole Parmer/Male lock 4-way polycarbonate stopcocks, pkg 10/K-30600-04). ![]() ![]() Luer 1-way stopcocks, at least 5 (Cole Parmer/Male lock 1-way polycarbonate stopcocks, pkg 10/K-30600-00).Luer 5-port manifold (Cole Parmer/ Polycarbonate manifold, 5 ports, pkg 10/ K-30600-43).Plastic 50 mL graduated cylinder (Fisher/Thermo Scientific Nalgene Polypropylene Cylinder 50mL/ 08-572C).We hope that additional alternatives will be added by other contributors over time. The list below is what was available and excellent at time of initiating this protocol. Note: there are many possible alternatives, and periodically, parts may become discontinued and exact replacements may or may not be available. The authors of that paper were those who designed the original version of this flow meter, which has been refined and improved since then to the version described in this protocol. Measurement of stem xylem hydraulic conductivity: field instrumentation and implementation, to be submitted. Melcher PJ, Holbrook NM, Burns MJ, Zwieniecki MA, Cobb AR, Brodribb TJ, Choat B, Sack L. The concepts and theory that form the background and other essential information is to be found in the paper, The data acquisition board is interfaced via USB cord to a computer (not shown). The transducers are powered by the black battery/charger in the background. The gray cables attached to the transducers on the flow meter run to the white data acquisition board on the right. The raised graduated cylinder in the background contains flow solution, that runs via tubing through the flow meter (shown raised on a petri dish) to the fitting in the foreground which is to be connected to the sample to be measured for hydraulic conductance. This is a relatively inexpensive, easily transportable and field deployable alternative to a 5-decimal analytical balance.įigure 1.The complete hydraulics floweter. This is a detailed protocol for constructing and operating a pressure-drop hydraulics flow meter that can be used for stem hydraulics, as described here, and with minimal changes, can be used for leaf hydraulics (evaporative flux method, or rehydration methods). University of California, Los Angeles Overview Lawren Sack, Megan Bartlett, Chris Creese, Gaëlle Guyot, Christine Scoffoni Author affiliations
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